Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

PPIH Protein (AA 1-177)

Dieses Recombinant PPIH-Protein wird in Escherichia coli (E. coli) produziert.
Produktnummer ABIN934991

Kurzübersicht für PPIH Protein (AA 1-177) (ABIN934991)

Target

Alle PPIH Proteine anzeigen
PPIH (Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH))

Protein-Typ

Recombinant

Biologische Aktivität

Active

Spezies

  • 6
  • 1
Human

Quelle

  • 4
  • 1
  • 1
  • 1
Escherichia coli (E. coli)

Applikation

SDS-PAGE (SDS)

Reinheit

> 95 % pure
  • Proteineigenschaft

    AA 1-177

    Sequenz

    MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM

    Produktmerkmale

    Purified recombinant Human PPIH protein
    Expression System: E.coli
    Bioactivity: Specific activity is > 40 nmoles/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-HCl pH 8.0 using chymotrypsin.
    Molecular weight on SDS-PAGE will appear higher.

    Endotoxin-Niveau

    < 1.0 EU per µg of protein (determined by LAL method)
  • Möchten Sie weitere Optionen für dieses Protein ?

    !
    Entdecken Sie unsere vordefinierten maßgeschneiderten Proteine und Proteinservices!

    Ihr Projekt erfordert weitere Anpassungen? Kontaktieren Sie uns und entdecken Sie unsere maßgeschneiderten Proteinlösungen

  • Applikationshinweise

    PPIH protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

    Testdurchführung

    1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
      2. Add recombinant Cyclophilin H protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
      3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
      4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
      5. Record the increase in A410 nm for 20 minutes

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    Supplied as a liquid in PBS, pH 7.4, containing 10 % glycerol.

    Handhabung

    Avoid repeated freeze/thaw cycles.

    Lagerung

    RT/-20 °C

    Informationen zur Lagerung

    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Target

    PPIH (Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH))

    Andere Bezeichnung

    PPIH

    Hintergrund

    Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. The cyclophilin H is a specific component of the human U4/U6 small nuclear ribonucleoprotein particle involved in the nuclear splicing of pre-mRNA. It stably associates with the U4/U6-60kD and -90kD proteins, the human orthologues of the Saccharomyces cerevisiae Prp4 and Prp3 splicing factors. Recombinant human cyclophilin H was expressed in E. coli and purified by conventional chromatography techniques.
    Alternative Names: Peptidylprolyl isomerase H CypH protein, EC 5.2.1.8 protein, PPIH protein, UsnRNP associated cyclophilin SnuCyp20 protein, Peptidylprolyl isomerase H protein, Cyclophilin H protein, , CYPH protein, MGC5016 protein, CYP20 protein, USACYP protein, PPIase H protein, Rotamase H protein, SnuCyp-20 protein, SnuCyp-20 protein, Small nuclear ribonucleoprotein particle-specific cyclophilin H protein

    Molekulargewicht

    19.2 kDa (177 AA)
Sie sind hier:
Chat with us!