GrpE Protein
Kurzübersicht für GrpE Protein (ABIN934953)
Target
Protein-Typ
Spezies
Quelle
Applikation
Reinheit
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Sequenz
- MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
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Produktmerkmale
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Purified recombinant E.coli GrpE protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. -
Endotoxin-Niveau
- < 1.0 EU per µg of protein (determined by LAL method)
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Applikationshinweise
- GrpE protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in 20 mM Tris, pH 8.0, containing 100 mM NaCl.
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Handhabung
- Avoid repeated freeze/thaw cycles.
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Lagerung
- RT/-20 °C
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Informationen zur Lagerung
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- GrpE
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Hintergrund
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GrpE ,co-chaperone of E. coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E. coli and purified by using the conventional column chromatography techniques.
Alternative Names: GrpE protein, HSP-70 cofactor protein, Heat shock protein B25.3 protein, HSP24 protein, HSP 70 cofactor. -
Molekulargewicht
- 21.8 kDa (197 AA)
Target
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