TXN Protein

Produktnummer ABIN934926

Produktdetails

Target: TXN (Thioredoxin (TXN))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Sequenz: MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV

Produktmerkmale: Purified recombinant Human Thioredoxin1 protein
Expression System: E.coli
Bioactivity: Specific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Molecular weight on SDS-PAGE will appear higher.

Reinheit: > 95 % pure

Anwendungsinformationen

Applikationshinweise: Thioredoxin1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Testdurchführung:

1. Prepare a 1 mL reaction cocktail in a suitable container. The final concentrations are 1 mM Tris, 61 mM sodium phosphate, 1 mM DTT, 0.1 % (w/v) insulin, 1 mM EDTA.
   2. Add 4 µg of recombinant thioredoxin into reaction cocktail and mix immediately.
   3. Incubate at 25 °C for 20 minutes.
   4. Record the increase in A650 nm for 15 minutes

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Supplied as a liquid in PBS, pH 7.4.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Antigendetails

Target: TXN (Thioredoxin (TXN))

Andere Bezeichnung: Thioredoxin1 (TXN Produkte)

Synonyme: TRDX Protein, TRX Protein, TRX1 Protein, ADF Protein, AW550880 Protein, Trx1 Protein, Txn Protein, trx Protein, TXN Protein, SCM1.18 Protein, trxA3 Protein, thioredoxin Protein, TR-1 Protein, Trx-1 Protein, thioredoxin Protein, thioredoxin 1 Protein, thioredoxin L homeolog Protein, similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544) Protein, thioredoxin pseudogene Protein, cytosolic thioredoxin Trx1 Protein, TXN Protein, Txn1 Protein, txn.L Protein, txn Protein, trx Protein, SCO0885 Protein, PHG25ORF099c Protein, LOC100349046 Protein, Txn Protein, trx1 Protein

Hintergrund: Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: ATL derived factor protein, TXN protein protein, Thioredoxin 1 protein, TR-I protein, TRX1 protein, TXN protein, TRX protein, , DKFZp686B1993 protein, Thioredoxin1, Surface associated sulphydryl protein protein, Thioredoxin protein, Thioredoxin -1 protein, Thioredoxin I protein, TRX 1 protein, Thioredoxin-2 protein, Thioredoxin-1 protein, ADF protein, SASP protein, Thioredoxin 1, TRDX protein, TRX2 protein, MGC61975 protein, Thioredoxin -1, Thioredoxin-1 protein

Molekulargewicht: 11.7 kDa (105 AA)

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis