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TXN Protein

TXN Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % pure SDS Active
Produktnummer ABIN934926
  • Target Alle TXN Proteine anzeigen
    TXN (Thioredoxin (TXN))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Spezies
    • 8
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 16
    • 13
    • 1
    • 1
    Escherichia coli (E. coli)
    Applikation
    SDS-PAGE (SDS)
    Sequenz
    MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV
    Produktmerkmale
    Purified recombinant Human Thioredoxin1 protein
    Expression System: E.coli
    Bioactivity: Specific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
    Molecular weight on SDS-PAGE will appear higher.
    Reinheit
    > 95 % pure
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    Discover our top product TXN Protein
  • Applikationshinweise
    Thioredoxin1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
    Testdurchführung
    1. Prepare a 1 mL reaction cocktail in a suitable container. The final concentrations are 1 mM Tris, 61 mM sodium phosphate, 1 mM DTT, 0.1 % (w/v) insulin, 1 mM EDTA.
      2. Add 4 µg of recombinant thioredoxin into reaction cocktail and mix immediately.
      3. Incubate at 25 °C for 20 minutes.
      4. Record the increase in A650 nm for 15 minutes
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Supplied as a liquid in PBS, pH 7.4.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    RT/-20 °C
    Informationen zur Lagerung
    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Target
    TXN (Thioredoxin (TXN))
    Andere Bezeichnung
    Thioredoxin1 (TXN Produkte)
    Hintergrund
    Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: ATL derived factor protein, TXN protein protein, Thioredoxin 1 protein, TR-I protein, TRX1 protein, TXN protein, TRX protein, , DKFZp686B1993 protein, Thioredoxin1, Surface associated sulphydryl protein protein, Thioredoxin protein, Thioredoxin -1 protein, Thioredoxin I protein, TRX 1 protein, Thioredoxin-2 protein, Thioredoxin-1 protein, ADF protein, SASP protein, Thioredoxin 1, TRDX protein, TRX2 protein, MGC61975 protein, Thioredoxin -1, Thioredoxin-1 protein
    Molekulargewicht
    11.7 kDa (105 AA)
    Pathways
    Carbohydrate Homeostasis, Cell RedoxHomeostasis
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