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DnaK (AA 1-384), (N-Term) Protein

Dieses Recombinant -Protein wird in Escherichia coli (E. coli) exprimiert.
Produktnummer ABIN934890

Kurzübersicht für DnaK (AA 1-384), (N-Term) Protein (ABIN934890)

Target

DnaK

Protein-Typ

Recombinant

Spezies

  • 5
  • 1
  • 1
Alle Spezies

Quelle

  • 7
Escherichia coli (E. coli)

Applikation

SDS-PAGE (SDS)

Reinheit

> 95 % pure
  • Proteineigenschaft

    AA 1-384, N-Term

    Sequenz

    MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTG

    Produktmerkmale

    Purified recombinant E.coli Dnak protein
    Expression System: E.coli
    Molecular weight on SDS-PAGE will appear higher.
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  • Applikationshinweise

    Dnak protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    Supplied as a liquid in 25 mM Tris-HCl, pH 7.5, containing 100 mM NaCl, 5 mM DTT, and 10 % glycerol.

    Konservierungsmittel

    Dithiothreitol (DTT)

    Vorsichtsmaßnahmen

    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Handhabung

    Avoid repeated freeze/thaw cycles.

    Lagerung

    RT/-20 °C

    Informationen zur Lagerung

    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Target

    DnaK

    Hintergrund

    DNAK, originally identified for its DNA replication by bacteriophage in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus.
    Alternative Names: dnaK protein, Heat shock 70 kDa protein protein, 1-384) protein, Co chaperone with DnaJ protein, Chaperone protein dnaK protein, HSP70 protein, Heat shock protein 70 protein, Dnak (N-term protein, ATPase binding domain protein, Chaperone Hsp70 protein,

    Molekulargewicht

    41.6 kDa (384 AA)
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