HIV-2 gp36 protein (His tag)

Produktnummer ABIN810024

Produktdetails

Target: HIV-2 gp36

Protein-Typ: Recombinant

Spezies: Human

Quelle: Yeast (Pichia pastoris)

Aufreinigungstag / Konjugat: His tag

Applikation: ELISA, Western Blotting (WB)

Produktmerkmale: Human Immunodeficiency Virus Type 2 (HIV-2) gp36, Recombinant ecto-domain of HIV-2 gp36. Contains a 6 histidine fusion partner. Reacts with HIV-2 positive human sera and monoclonal antibodies as determined by ELISA and Western blot. Major immunospecific band at 14 kDa, minor bands at 5, 16, 18, 28 and 42 kDa.

Aufreinigung: Purity determined by SDS-PAGE and Western blot

Anwendungsinformationen

Applikationshinweise: Suitable for use in ELISA and Western blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. HIV-2 gp36 ELISA with HIV-2 positive sera at 1:2.000 with 1-100 nanograms per well gp36. Background with BSA or normal sera OD405 <0.1. Alkaline phosphatase substrate for 45 min.

Kommentare:

Infectious Disease Antigens and Toxins

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 6 M Urea, 0.02 M Tris-HCl, 0.5 M Sodium chloride, pH 7.0 to 8.0 at room temperature

Konservierungsmittel: Without preservative

Handhabung: Aliquot to avoid multiple freeze thaws.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -70 °C or below.

Antigendetails

Target: HIV-2 gp36

Substanzklasse: Viral Protein

Molekulargewicht: 14 kDa