Vimentin Protein (VIM) (AA 1-466)

Produktnummer ABIN8057080

Dieses Recombinant Vimentin-Protein wird in Mammalian Cells exprimiert.

Kurzübersicht für Vimentin Protein (VIM) (AA 1-466) (ABIN8057080)

Target: Vimentin (VIM)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Mammalian Cells

Applikation: Immunogen (Imm)

Reinheit: > 85 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-466

Verwendungszweck: Recombinant Human VIM Protein(Halo Tag)

Sequenz: Met1-Glu466

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: Vimentin (VIM)

Andere Bezeichnung: VIM

Hintergrund: Vimentin,Vimentin is a type III intermediate filament(IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl(tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.

Molekulargewicht:

Calculated MW: 93.1 kDa

Observed MW: 91 kDa

Gen-ID: 7431

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose