IFN alpha 2b Protein (AA 24-188)

Produktnummer ABIN8056968

Dieses Recombinant IFN alpha 2b-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für IFN alpha 2b Protein (AA 24-188) (ABIN8056968)

Target: IFN alpha 2b

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Cell Culture (CC)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 24-188

Verwendungszweck: Recombinant Human IFNα2b/IFNA2 Protein

Sequenz: Cys24-Glu188

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV) The ED₅₀ for this effect is 5 ng/mL.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: IFN alpha 2b

Andere Bezeichnung: IFNalpha2b

Hintergrund: IFN-Alpha-2,IFN-alpha 2b,IFNA2,Interferon Alpha-2,Interferon Alpha-A,LeIF A,At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end.

Molekulargewicht:

Calculated MW: 19.4 kDa

Observed MW: 17 kDa

Gen-ID: 3440

UniProt: P01563