UBE2A Protein

Produktnummer ABIN7939675

Dieses Recombinant UBE2A-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für UBE2A Protein (ABIN7939675)

Target: UBE2A (ube2a) (Ubiquitin-Conjugating Enzyme E2A (ube2a))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant UBE2A protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant UBE2A protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: UBE2A (ube2a) (Ubiquitin-Conjugating Enzyme E2A (ube2a))

Andere Bezeichnung: UBE2A

Hintergrund:

Protein Background: UBE2A (Ubiquitin-conjugating enzyme E2 A) also known as HHR6A,MRXS30, MRXSN, RAD6A, and UBC. UBE2A is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2A can accept ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for post replication repair of UV-damaged DNA.

Short Description: Recombinant UBE2A protein that includes full length of human UBE2A protein (accession number NP_003327.2) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 19.48 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.

Aliases: Ubiquitin conjugating enzyme E2 A, HHR6A, MRXS30, MRXSN, RAD6A, UBC