PIM2 Protein

Produktnummer ABIN7939489

Recombinant PIM2-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für PIM2 Protein (ABIN7939489)

Target: PIM2 (Proto-Oncogene Pim-2 (Serine Threonine Kinase) (PIM2))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant PIM2 protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant PIM2 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: PIM2 (Proto-Oncogene Pim-2 (Serine Threonine Kinase) (PIM2))

Andere Bezeichnung: PIM2

Hintergrund:

Protein Background: PIM2 (Pim-2 Proto-Oncogene, Serine/Threonine Kinase) is a Protooncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Its oncogenic activity exerts through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Pim2 regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. It also mediates survival signaling through phosphorylation of BAD, which induces release of the antiapoptotic protein Bcl-X(L)/BCL2L1. PIM2 promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade, this process requires phosphorylation of MAP3K8/COT. It promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B and involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate. Diseases associated with PIM2 include Colorectal Adenocarcinoma and Lymphoma.

Short Description: Recombinant PIM2 protein was expressed in baculovirus expression system as the full length protein (accession number NP_006866.2) with a N-terminal FLAG tag. The molecular weight of the protein is 35.5 kDa.

Aliases: Pim-2 Proto-Oncogene, Serine/Threonine Kinase, Pim-2h