PKC eta Protein

Produktnummer ABIN7939470

Dieses Recombinant PKC eta-Protein wird in Baculovirus infected Insect Cells produziert.

Kurzübersicht für PKC eta Protein (ABIN7939470)

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant PRKCH protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant PRKCH protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Andere Bezeichnung: PRKCH

Hintergrund:

Protein Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCH (Protein Kinase C Eta), a calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. PRKCH can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). Diseases associated with PRKCH include Stroke, Ischemic and Glioblastoma.

Short Description: Recombinant PRKCH protein was expressed in baculovirus expression system as the full length protein (accession number NP_006246.2) with a N-terminal FLAG tag. The molecular weight of the protein is 79.1 kDa.

Aliases: Protein Kinase C Eta, PKC Eta

Pathways: Myometrial Relaxation and Contraction, Thromboxane A2 Receptor Signaling