PRDM16 Protein

Produktnummer ABIN7939441

Dieses Recombinant PRDM16-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für PRDM16 Protein (ABIN7939441)

Target: PRDM16 (PR Domain Containing 16 (PRDM16))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant PRDM16 protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant PRDM16 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: PRDM16 (PR Domain Containing 16 (PRDM16))

Andere Bezeichnung: PRDM16

Hintergrund:

Protein Background: PRDM16 (Histone-lysine N-methyltransferase PRDM16), also known as CMD1LL, KMT8F, LVNC8, MEL1 and PFM13. PRDM16 was found in t(1,3)(p36,q21)-positive AML, which has similar clinicopathological features to3q21q26 syndrome, as a transcriptionally activated gene near the chromosomal breakpoint. The structure of the PRDM16 gene product is very similar to that of MDS1/EVI1. Both products have the PRdomain at the amino-terminus, 2 separated DNA binding domains with 7 and 3 tandem zinc finger repeats, CtBP-binding motifs, and other possible transcriptional regulatory domains. By similarity, PRDM16 probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation.

Short Description: Recombinant PRDM16 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_071397.3) with an N-terminal FLAG tag. The molecular weight of the protein is 141.5 kDa.

Aliases: Transcription Factor MEL1, KMT8F, Histone-Lysine N-methyltransferase PRDM16, CMD1LL, KMT8F, LVNC8, MEL1, PFM13

Pathways: Stem Cell Maintenance, Brown Fat Cell Differentiation