LCK Protein

Produktnummer ABIN7939235

Recombinant LCK-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für LCK Protein (ABIN7939235)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant LCK protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant LCK protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Andere Bezeichnung: LCK

Hintergrund:

Protein Background: LCK (LCK Proto-Oncogene, Src Family Tyrosine Kinase) is a non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. It plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.

Short Description: Recombinant LCK protein was expressed in baculovirus expression system as the full length protein (accession number NP_001036236.1) with a N-terminal FLAG tag. The molecular weight of the protein is 59.3 kDa.

Aliases: LCK Proto-Oncogene, Src Family Tyrosine Kinase

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling