Histone 3 Protein (H3K4me3, Nucleosome)

Produktnummer ABIN7939073

Dieses Recombinant Histone 3-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Histone 3 Protein (H3K4me3, Nucleosome) (ABIN7939073)

Target: Histone 3 (H3) (Histone H3 (H3))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Screening Assay (ScA), Enzyme Kinetics (EK)

Produktdetails

Proteineigenschaft: H3K4me3, Nucleosome

Verwendungszweck: Recombinant Mononucleosomes H3K4me3 (EPL) - biotinylated

Produktmerkmale: Nucleosomes comprise the smallest subunit of chromatin, they consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for use in in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.

Aufreinigung: purified using FPLC.

Anwendungsinformationen

Applikationshinweise: Recombinant Mononucleosomes H3K4me3 (EPL) - biotinylated is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant Mononucleosomes H3K4me3 - biotinylated (20 μg protein + 20 μg DNA) are supplied in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT and 20 % glycerol.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund:

Protein Background: In vivo, histones are wrapped around by DNA in chromatin. Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.

Short Description: Recombinant Mononucleosomes H3K4me3 (EPL) - biotinylated, consist of 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NP_003503.1), H2B that includes amino acids 1-126 (end) (accession number NP_003509.1), H3.2 that includes amino acids 1-136 (end) (accession number NP_066403.2) with trimethylation at lysine 4, and H4 that includes amino acids 1-103 (end) (accession number NP_003539.1). All of these histones were expressed in E. coli cells. The molecular weight of histone octamer is ~108 kDa. H3K4me3 (Histone H3 trimethyl Lys4) proteins are generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to a N-terminal histone tail peptide containing trimethyl lysine 4 via a native peptide bond.

Aliases: methylated Histone, H3K4 tri-methylated, EPL Histone