Histone H3.3 Protein (G34R, Nucleosome) (Biotin)

Produktnummer ABIN7938945

Dieses Recombinant Histone H3.3-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Histone H3.3 Protein (G34R, Nucleosome) (Biotin) (ABIN7938945)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Screening Assay (ScA), Enzyme Kinetics (EK)

Produktdetails

Proteineigenschaft: G34R, Nucleosome

Aufreinigungstag / Konjugat: Dieses Histone H3.3 Protein ist gelabelt mit Biotin.

Verwendungszweck: Recombinant Mononucleosomes H3.3 (G34R) - biotin

Produktmerkmale: Recombinant Mononucleosomes H3.3 (G34R) - biotinylated consist of a 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NP_003503.1), H2B that includes amino acids 1-126 (end) (accession number NP_003509.1), H3.3 that includes amino acids 1-136 (end) (accession number NP_002098.1) with a point mutation Gly34Arg, and H4 that includes amino acids 1-103 (end) (accession number NP_003539.1). All of these histones were expressed in E. coli. The molecular weight of histone octamer is ~108 kDa. The recombinant protein is ≥95 % by SDS-PAGE gel. Nucleosomes comprise the smallest subunit of chromatin, they consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for use in in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.

Anwendungsinformationen

Applikationshinweise: Recombinant Mononucleosomes H3.3 (G34R) - biotinylated are suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Recombinant Mononucleosomes H3.3 (G34R) - biotinylated (20 μg protein + 20 μg DNA) is supplied at a protein concentration of 0.8 μg/ul in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT and 20 % glycerol.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Andere Bezeichnung: Histone H3.3

Hintergrund:

Protein Background: In vivo, the nucleosome is the basic structural unit of chromatin. It consists of about 146 bp of DNA wrapped around a core of eight histones of four different types: H2A, H2B, H3 and H4. Histones are subject to posttranslational modifications, such as methylation, acetylation, phosphorylation, mono-ubiquitination, tec. Histone modifications influence multiple chromatin templated processes such as gene transcription, DNA repair and recombination. Besides the ""major"" histones, there are some histone variants in specific regions of chromatin or in specific cell types. Histone variants were involved in multiple biology processes including chromosome segregation, DNA repair, transcriptional regulation and mRNA processing. Histone H3.3 point mutations (K27 and G34) are found in 1/3 of pediatric glioblastomas. Up to 78 % of diffuse intrinsic pontine gliomas (DIPGs) carry K27M and 36 % of non-brainstem gliomas carry either K27M or G34R/V mutations. High-frequency mutations in histone H3 include K36M in chondroblastomas and G34W/L in giant cell tumors of bone, diseases of adolescents and young adults. Histone H3.3 mutations drive pediatric glioblastoma through upregulation of MYCN. Nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.

Short Description: Recombinant Mononucleosomes H3.3 (G34R) - biotinylated consist of a 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NP_003503.1), H2B that includes amino acids 1-126 (end) (accession number NP_003509.1), H3.3 that includes amino acids 1-136 (end) (accession number NP_002098.1) with a point mutation Gly34Arg, and H4 that includes amino acids 1-103 (end) (accession number NP_003539.1). All of these histones were expressed in E. coli cells. The molecular weight of histone octamer is ~108 kDa. The recombinant protein is ≥95 % by SDS-PAGE gel.

Molekulargewicht: 108 kDa

NCBI Accession: NP_003503