K-RAS Protein (A146T, AA 1-186)

Produktnummer ABIN7938891

Dieses Recombinant K-RAS-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für K-RAS Protein (A146T, AA 1-186) (ABIN7938891)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Screening Assay (ScA), Functional Studies (Func), Activity Assay (AcA)

Produktdetails

Proteineigenschaft: A146T, AA 1-186

Verwendungszweck: Recombinant KRAS (1-186, A146T) protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant KRAS (1-186, A146T) protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Andere Bezeichnung: KRAS

Hintergrund:

Protein Background: KRAS (GTPase KRas) protein is a GDP/GTP binding protein, and the more important genes of the same family include HRAS and NRAS. KRAS is activated when combined with GTP and closed when combined with GDP. KRAS can be transiently activated by growth factors or tyrosine kinases (such as EGFR). The activated KRAS can activate downstream signals such as PI3K-AKT-mTOR that control cell production pathway, and the RAS-RAF-MEK-ERK signaling pathway that controls cell proliferation. Mutant KRAS will continue to be activated even without activation of kinases such as EGFR, resulting in continued cell proliferation and eventually canceration. KRAS mutations are found in a variety of tumors, the most common ones include lung cancer and pancreatic cancer.

Short Description: Recombinant KRAS (1-186, A146T) protein that includes amino acids 1-186 of human KRAS protein (accession number NP_203524.1) was expressed in E. coli cells with a A146T mutation and a C-terminal 6xHis tag. The molecular weight of the protein is 22.36 kDa.

Aliases: GTPase KRas, KRAS1, KRAS2, Kirsten Rat Sarsoma Viral Oncogene Homolog, C-Ki-Ras, KRAS3B, K-Ras P21 Protein