Fibrillarin Protein (FBL)

Produktnummer ABIN7938842

Recombinant Fibrillarin-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Fibrillarin Protein (FBL) (ABIN7938842)

Target: Fibrillarin (FBL)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant FBL protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant FBL protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: Fibrillarin (FBL)

Andere Bezeichnung: FBL

Hintergrund:

Protein Background: FBL(rRNA 2'-O-methyltransferase fibrillarin) is a S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins.Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA.Site specificity is provided by a guide RNA that base pairs with the substrate.Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA .Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes.U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity .Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus.

Short Description: Recombinant FBL protein that includes full length of human FBL protein (accession number NP_001427.2) was expressed was expressed in E. coli cells with an N-terminal GST tag. The molecular weight of the protein is 60.62 kDa.

Aliases: rRNA 2'-O-methyltransferase fibrillarin, FLRN, RNU3IP1, Nop1, FIB, EC 2.1.1.37

Pathways: Ribonucleoside Biosynthetic Process