EIF4A1 Protein

Produktnummer ABIN7938829

Recombinant EIF4A1-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für EIF4A1 Protein (ABIN7938829)

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant EIF4A1 protein

Produktmerkmale: EIF4A1 (Eukaryotic Translation Initiation Factor 4A, Isoform 1), also called as EIF4A or DDX2A, is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome, ubiquitous with high expression in skeletal muscle and ovary. The helicase activity of EIF4A1 and function in translation are inhibited by interaction with PDCD4.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant EIF4A1 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 20 % glycerol, and 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Andere Bezeichnung: EIF4A1

Hintergrund:

Protein Background: EIF4A1 (Eukaryotic Translation Initiation Factor 4A, Isoform 1), also called as EIF4A or DDX2A, is a ATP dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome,ubiquitous with high expression in skeletal muscle and ovary. The helicase activity of EIF4A1 and function in translation are inhibited by interaction with PDCD4.

Short Description: Recombinant EIF4A1 protein was expressed in E.coli as the full length protein (accession number NP_001407.1) with an N-terminal 6xHis Tag. The molecular weight of the protein is 49.7 kDa.

Aliases: Eukaryotic Translation Initiation Factor 4A1, DDX2A