EEF2 Protein

Produktnummer ABIN7938823

Recombinant EEF2-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für EEF2 Protein (ABIN7938823)

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant EEF2 protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Recombinant EEF2 protein is in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Andere Bezeichnung: EEF2

Hintergrund:

Protein Background: EEF2 (Elongation factor 2) promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. Mostly eEF2 is regulated by phosphorylation and dephosphorylation by a specific kinase known as eEF2 kinase, which itself is up regulated by various mechanisms in the eukaryotic cell. The activity of this kinase is dependent on calcium ions and calmodulin. Recently it has been shown that the activity of eEF2 kinase is regulated by MAP kinase signalling and mTOR signalling pathway.

Short Description: Recombinant EEF2 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001952.1) with an N-terminal FLAG tag. The molecular weight of EEF2 is 96.91 kDa.

Aliases: EF2, Elongation Factor 2, EF-2, Epididymis Secretory Binding Protein

Pathways: AMPK Signaling