eEF1A1 Protein

Produktnummer ABIN7938816

Dieses Recombinant eEF1A1-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für eEF1A1 Protein (ABIN7938816)

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant EEF1A1 protein

Produktmerkmale: EEF1A1 (Eukaryotic Translation Elongation Factor 1 Alpha 1), also called as LENG7, CTCL Tumor Antigen or CCS3, is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. EEF1A1 is identified as an autoantigen in 66 % of patients with Felty syndrome. EEF1A1 can promote the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, it can form a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. It may also mediate accelerated synthesis of free thiol-containing proteins in response to HC-induced oxidative stress by homocysteine (HC).

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant EEF1A1 protein is supplied in 25 mM Tris-HCl pH 7.4, 500 mM NaCl, 10 % glycerol, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Andere Bezeichnung: EEF1A1

Hintergrund:

Protein Background: EEF1A1 (Eukaryotic Translation Elongation Factor 1 Alpha 1), also called as LENG7, CTCL Tumor Antigen or CCS3, is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. EEF1A1 is identified as an autoantigen in 66 % of patients with Felty syndrome. EEF1A1 can promote the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, it can form a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. It may also mediate accelerated synthesis of free thiol-containing proteins in response to HC-induced oxidative stress by homocysteine (HC).

Short Description: Recombinant EEF1A1 protein was expressed in E.coli as the full length protein (accession number NP_001393.1) with an N-terminal 6xHis Tag. The molecular weight of the protein is 53.7 kDa.

Aliases: Eukaryotic Translation Elongation Factor 1 Alpha 1