DAPK3 Protein

Produktnummer ABIN7938757

Recombinant DAPK3-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für DAPK3 Protein (ABIN7938757)

Target: DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant DAPK3 / ZIPK protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant DAPK3 / ZIPK protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))

Andere Bezeichnung: DAPK3 & ZIPK

Hintergrund:

Protein Background: DAPK3 (Death Associated Protein Kinase 3), a serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. This protein is involved in the regulation of smooth muscle contraction and regulates both type I (caspase-dependent) apoptotic and type II (caspaseindependent) autophagic cell deaths signal, depending on the cellular setting. DAPK3 is involved in regulation of starvation-induced autophagy and regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, DAPK3 regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. DAPK3 phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B.

Short Description: Recombinant DAPK3 / ZIPK protein was expressed in baculovirus expression system as the full length protein (accession number NP_001339.1) with a N-terminal FLAG tag. The molecular weight of the protein is 53.8 kDa.

Aliases: Death Associated Protein Kinase 3, DAP Kinase 3, ZIPK, Ziper interacting protein kinase