BRD2 Protein

Produktnummer ABIN7938621

Dieses Recombinant BRD2-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für BRD2 Protein (ABIN7938621)

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Screening Assay (ScA), Activity Assay (AcA), Functional Studies (Func)

Produktdetails

Verwendungszweck: Recombinant BRD2 (BD1+BD2) protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant BRD2 (BD1+BD2) protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 10 % glycerol, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Andere Bezeichnung: BRD2

Hintergrund:

Protein Background: Bromodomain-containing protein 2 (BRD2), also known as RING3, belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female sterile homeotic protein gene in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD2 causes elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. BRD2 has been shown to interact with E2F1 and with histone H4 acetylated at Lys12 via its two bromodomains. BRD2 may play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses.

Short Description: The protein corresponding to amino acids 71-455 that contains the bromodomain sequence of BRD2 (accession number NP_001106653.1) was expressed in E. coli and contains an N-terminal His tag with an observed molecular weight of 45.51 kDa. It shows binding specificity for acetylated H4K5, H4K5/8, H4K5/12, H4K8/12, H4K12/16, H4K12/16/20 and H4K5/8/12/16

Aliases: FSH, NAT, RNF3, FSRG1, RING3, D6S113E

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion