alpha-Dendrotoxin (Active) protein (Atto 647N)

Produktnummer ABIN7885642

Dieses Native alpha-Dendrotoxin-Protein wird in Dendroaspis angusticeps (Eastern green mamba) produziert.

Kurzübersicht für alpha-Dendrotoxin (Active) protein (Atto 647N) (ABIN7885642)

Target: alpha-Dendrotoxin

Protein-Typ: Native

Biologische Aktivität: Active

Spezies: Dendroaspis angusticeps

Quelle: Dendroaspis angusticeps (Eastern green mamba)

Applikation: Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)

Produktdetails

Aufreinigungstag / Konjugat: Atto 647N

Verwendungszweck: α-Dendrotoxin-ATTO Fluor-647N

Sequenz: QPRRKLCILH RNPGRCYDKI PAFYYNQKKK QCERFDWSGC GGNSNRFKTI EECRRTCIG-OH

Spezifität: Toxin target: K_V1.1, K_V1.2, K_V1.6,ASIC channels

Produktmerkmale: ATTO 647N (Ex/Em: 646/664 nm). The extent of labeling is 1-2 Molecules of dye per α-Dendrotoxin molecule.

Biological Activity Comment: α-Dendrotoxin is a potent and selective blocker of neuronal K_V1.1, K_V1.2, K_V1.6 potassium channels.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary.

Note: The product is typically supplied in a 0.5 mL conical vial.  

The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH₂O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer.

Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handhabung: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light

Lagerung: -20 °C

Informationen zur Lagerung: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.

Antigendetails

Target: alpha-Dendrotoxin

Hintergrund: A fluorescent probe for imaging and sensitive detection of K_v1.1, K_v1.2, and K_v1.6 channels.

Molekulargewicht: ~7990 Da