Osteopontin Protein (AA 22-323)

Produktnummer ABIN7866059

Recombinant Osteopontin-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Osteopontin Protein (AA 22-323) (ABIN7866059)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: >95 % , as determined by SDS-PAGE and HPLC

Produktdetails

Proteineigenschaft: AA 22-323

Verwendungszweck: Human SPP1 Protein Recombinant

Sequenz: VHHPPSYVAH LASDFGVRVF QQVAQASKDR NVVFSPYGVA SVLAMLQLTT GGETQQQIQA AMGFKIDDKG MAPALRHLYK ELMGPWNKDE ISTTDAIFVQ RDLKLVQGFM PHFFRLFRST VKQVDFSEVE RARFIINDWV KTHTKGMISN LLGKGAVDQL TRLVLVNALY FNGQWKTPFP DSSTHRRLFH KSDGSTVSVP MMAQTNKFNY TEFTTPDGHY YDILELPYHG DTLSMFIAAP YEKEVPLSAL TNILSAQLIS HWKGNMTRLP RLLVLPKFSL ETEVDLRKPL ENLGMTDMFR QFQADFTSLS DQEPLHVAQA LQKVKIEVNE SGTVASSSTA VIVSARMAPE EIIMDRPFLF VVRHNPTGTV LFMGQVMEP

Produktmerkmale:

Protein Size: Recombinant SPP1 migrates as an approximately 42 kDa protein under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human SPP1 mature chain was expressed in E. Coli. Recombinant Human C-type lectin domain family 11 member A encodes amino acid residues 22-323.

Endotoxin-Niveau: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37°C.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: SPP1 was Lyophilized from PBS, pH 7.2.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 24 months

Antigendetails

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Andere Bezeichnung: SPP1

Molekulargewicht: 42 kDa

Pathways: Regulation of Cell Size