FAM131B-BRAF (Active) protein (GST tag)

Produktnummer ABIN7856311

Dieses Recombinant FAM131B-BRAF-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für FAM131B-BRAF (Active) protein (GST tag) (ABIN7856311)

Target: FAM131B-BRAF

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Kinase Activity Assay (KAA)

Produktdetails

Aufreinigungstag / Konjugat: GST tag

Verwendungszweck: Recombinant FAM131B-BRAF (Fex2Bex9), Active

Sequenz: FAB131B (1-30 exon2)-BRAF (381-end or exon9-18)

Produktmerkmale: Recombinant human fusion FAB131B (1-30 exon2)-BRAF (381-end or exon9-18) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Sterilität: Sterile

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25-1.0 mM DTT, 0-0.1 mM PMSF, 10-25 % glycerol

Konservierungsmittel: Dithiothreitol (DTT), PMSF

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

Haltbarkeit: 12 months

Antigendetails

Target: FAM131B-BRAF

Sub Type: Fusionprotein

Hintergrund: KIAA1549-BRAF is a gene fusion resulting from a tandem duplication event involving the BRAF kinase gene that have recently been identified as the most frequent genetic alteration in many cancers. The KIAA1549-BRAF fusion typically results from a 2.0 Mb tandem duplication in chromosome band 7q34. The KIAA1549:BRAF fusion gene is considered a driver genetic event in pilocytic astrocytoma and many other pediatric brain neoplasms. KIAA1549-BRAF fusion gene and BRAF (V600E) mutation may be responsible for deregulation of the Ras-RAF-ERK signaling pathway in many brain cancers.

Molekulargewicht: ~75 kDa

NCBI Accession: NM_004333