EML4-ALK (Active) protein (GST tag)

Produktnummer ABIN7856245

Dieses Recombinant EML4-ALK-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für EML4-ALK (Active) protein (GST tag) (ABIN7856245)

Target: EML4-ALK

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Kinase Activity Assay (KAA)

Produktdetails

Aufreinigungstag / Konjugat: GST tag

Verwendungszweck: Recombinant EML4-ALK (Eex14Aex20), Active

Sequenz: EML4 (1-534) - ALK (1058-end)

Produktmerkmale: Recombinant human fusion protein EML4 (1-534a.a., exons1-14) - ALK (1058-end, exons20-29) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Sterilität: Sterile

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25-1.0 mM DTT, 0-0.1 mM PMSF, 10-25 % glycerol

Konservierungsmittel: Dithiothreitol (DTT), PMSF

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

Haltbarkeit: 12 months

Antigendetails

Target: EML4-ALK

Sub Type: Fusionprotein

Hintergrund: ALK or CD246 is a receptor tyrosine kinase, which belongs to the insulin receptor superfamily which comprises an extracellular domain, a hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. CD246 plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. ALK-positive neoplasms represent a distinct entity because the morphology of the tumors is often neither anaplastic nor large cell and the tumors should be referred to as ALK lymphomas. CD246 has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. EML4-ALK mutations in lung cancer confer resistance to ALK inhibitors.

Molekulargewicht: ~152 kDa

NCBI Accession: NM_019063, NM_004304