IgE Protein (Biotin)
Kurzübersicht für IgE Protein (Biotin) (ABIN7827980)
Target
Alle IgE Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Aufreinigungstag / Konjugat
- Dieses IgE Protein ist gelabelt mit Biotin.
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Spezifität
- Binds with Fc effector domain to human and non human primate IgE receptors, no cross-reactivity with rodent or canine IgE receptors, specific antigen unknown
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Sensitivität
- Reactivity > 99 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone SUS-11)
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Produktmerkmale
- IGHE, chromosome 14q32.33 Source: Human B cell hybridoma from healthy donors (CD40 cell culture system)
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Aufreinigung
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - mouse anti-human IgE mAb (BSW17)
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IgE (AA 209-428) protein (His-Avi Tag)
Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)Spezies: Cynomolgus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 91-421) (Active) protein (His tag)Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 198-421) (Active) protein (His-Avi Tag)Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)Spezies: Human Wirt: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, FACS
IgE (AA 1-429) protein (His tag)Spezies: Ratte Wirt: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-428) protein (His tag)Spezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-432) protein (His tag)Spezies: Cynomolgus Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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Applikationshinweise
- For functional cellular assays (mast cell / basophil activation)
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1 mg/mL
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Buffer
- PBS, pH=7.2, azide free
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Konservierungsmittel
- Azide free
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Handhabung
- Aliquot to Avoid repeated freezing and thawing.
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Lagerung
- 4 °C
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- IgE
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Hintergrund
- Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA, FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FceRIa), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells
Target
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