Apelin Receptor Protein-VLP (APLNR) (AA 1-380)

Produktnummer ABIN7827854

VLP Apelin Receptor-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für Apelin Receptor Protein-VLP (APLNR) (AA 1-380) (ABIN7827854)

Target: Apelin Receptor (APLNR)

Protein-Typ: VLP

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 1-380

Verwendungszweck: Human APLNR VLP

Sequenz: Met1-Asp380

Produktmerkmale: Recombinant Human APLNR VLP is expressed from HEK293.It contains Met1-Asp380 (It may have cross reaction with anti-His antibody).

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human APLNR VLP at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-APLNR Antibody, hFc Tag with the EC50 of 0.20ug/ml determined by ELISA (QC Test).,The purity of Human APLNR VLP is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Kommentare:

Virus-like particles (VLPs) are formed from the outer capsid protein of a virus and are tiny nanoparticles formed by the automatic assembly of one or more capsid proteins. VLPs do not contain viral infectious genomes, so they are relatively safe during production operations. The SAMS™ protein engineering platform has been used to express a series of biotinylated, non-biotinylated, and fluorescently-labeled VLP-displayed antigens. They are suitable for SPR, ELISA, CAR-T positive rate detection, and other experimental scenarios.

Virus-Like Particles (VLPs) are highly immunogenic, meaning that they can elicit a strong immune response in the host. VLPs are recognized by the immune system and are taken up by antigen-presenting cells (APCs) such as dendritic cells. Once taken up by APCs, VLPs are processed and presented to T cells, which can trigger the activation of B cells to produce antibodies against the displayed antigen. Because VLPs resemble the structure and composition of native viruses, they are highly effective at inducing both humoral and cellular immune responses.

Generally, VLPs range in size from approximately 20 to 200 nanometers (nm). Compared to a cell-based immunization approach, their smaller size can optimize the immune response to target the specific antigen displayed on the surface of the engineered VLPs.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4).

Lagerung: -80 °C

Informationen zur Lagerung: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Apelin Receptor (APLNR)

Andere Bezeichnung: APLNR

Hintergrund: Apelin receptor (APJ), a member of the class A family of G protein-coupled receptor (GPCR), plays a crucial role in regulating cardiovascular and central nervous systems function. APJ influences the onset and progression of various diseases such as hypertension, atherosclerosis, and cerebral stroke, making it an important target for drug development.

Molekulargewicht: 46.7 kDa.

UniProt: P35414