TH Protein (His tag)

Produktnummer ABIN7669818

Recombinant TH-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für TH Protein (His tag) (ABIN7669818)

Target: TH

Protein-Typ: Recombinant

Biologische Aktivität: Inactive

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 %

Produktdetails

Aufreinigungstag / Konjugat: Dieses TH Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse TH protein (His tag)

Sequenz: Phe 34-Leu 301

Aufreinigung: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 10 EU/mg of the protein as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: TH

Hintergrund: Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.,DYT14,DYT5b,TH,TY3H,TYH,The,Tyrosine 3 hydroxylase,YH

Molekulargewicht:

calculated_mw: 29.4 kDa

observed_mw: 32 kDa

UniProt: P24529