NOV Protein (Fc Tag)

Produktnummer ABIN7669350

Recombinant NOV-Protein exprimiert in Mammalian Cells.

Kurzübersicht für NOV Protein (Fc Tag) (ABIN7669350)

Target: NOV (Nephroblastoma Overexpressed (NOV))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Mammalian Cells

Applikation: Immunogen (Imm)

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses NOV Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Recombinant Mouse NOV/CCN3 Protein(Fc Tag)

Sequenz: Gln22-lle354

Aufreinigung: SDS-PAGE analysis of Mouse NOV/CCN3 proteins, 2 μg/lane of Recombinant Mouse NOV/CCN3 proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 80 KD

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: NOV (Nephroblastoma Overexpressed (NOV))

Andere Bezeichnung: NOV

Hintergrund: CCN,CCN family member,NovH,CCN family member 3,Nephroblastoma-overexpressed gene protein homolog,Protein NOV homolog,Nov,Ccn3,Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is a putative ligand for integrin receptors, is tightly associated with the extracellular matrix, and mediates diverse cellular functions, including cell adhesion and proliferation. CCN3 has been shown to negatively regulate growth althoμgh it promotes migration in a cell type-specific manner. This secreted protein belongs to the CCN family, and its expression was observed in a broad variety of tissues from the early stage of development, and altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. Mature CCN3 protein has five distinct modules and truncated protein variants with altered function are found in many cancers. CCN3 acts throμgh the core stem cell signaling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of some cell lineages including hematopoietic, osteogenic, and chondrogenic. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis, and a crucial component within the bone marrow microenvironment.

Molekulargewicht:

Calculated MW: 61.5 kDa

Observed MW: 80 kDa

UniProt: Q64299

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding