IgE Protein (AA 1-424) (His tag)
Kurzübersicht für IgE Protein (AA 1-424) (His tag) (ABIN7668917)
Target
Alle IgE Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-424
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Aufreinigungstag / Konjugat
- Dieses IgE Protein ist gelabelt mit His tag.
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Verwendungszweck
- Recombinant Porcine IGHE Protein (His Tag)
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Sequenz
- Ser1-Lys424
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Sterilität
- 0.2 μm filtered
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Endotoxin-Niveau
- < 1.0 EU/mg of the protein as determined by the LAL method
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Biological Activity Comment
- Not validated for activity
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IgE (AA 209-428) protein (His-Avi Tag)
Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)Spezies: Cynomolgus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 91-421) (Active) protein (His tag)Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 198-421) (Active) protein (His-Avi Tag)Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)Spezies: Human Wirt: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, FACS
IgE (AA 1-429) protein (His tag)Spezies: Ratte Wirt: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-432) protein (His tag)Spezies: Cynomolgus Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-428) protein (His tag)Spezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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Buffer
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Haltbarkeit
- 12 months
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- IgE
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Andere Bezeichnung
- IGHE
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Substanzklasse
- Antibody
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Hintergrund
- IGHE,IgE heavy chain constant region,IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies-and the life threatening respiratory distress of asthma and anaphylactic shock.
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Molekulargewicht
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Calculated MW: 46.5 kDa
Observed MW: 60-70 kDa
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UniProt
- K7ZJQ0
Target
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