HFM1 Protein (AA 5325-5925) (His-MBP)

Produktnummer ABIN7668895

Dieses Recombinant HFM1-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für HFM1 Protein (AA 5325-5925) (His-MBP) (ABIN7668895)

Target: HFM1 (HFM1, ATP-Dependent DNA Helicase Homolog (HFM1))

Protein-Typ: Recombinant

Biologische Aktivität: Inactive

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: Escherichia coli (E. coli)

Reinheit: > 80 %

Produktdetails

Proteineigenschaft: AA 5325-5925

Aufreinigungstag / Konjugat: Dieses HFM1 Protein ist gelabelt mit His-MBP.

Verwendungszweck: Recombinant SARS-CoV-2 Helicase Protein (His Tag)

Sequenz: Ala5325-Gln5925

Aufreinigung: > 80 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Antigendetails

Target: HFM1 (HFM1, ATP-Dependent DNA Helicase Homolog (HFM1))

Andere Bezeichnung: Helicase

Hintergrund: The non-structural protein 13 (nsp13) of SARS-CoV 2 is a helicase that separates double-stranded RNA or DNA with a 5'-3' polarity, using the energy of nucleotide hydrolysis. A basic biochemical characterization of nsp13 demonstrated that it can unwind both doublestranded DNA and RNA in a 5'-3' direction, and it can hydrolyze all deoxyribonucleotide and ribonucleotide triphosphates. Helicases are motor proteins that utilize the energy derived from nucleotide hydrolysisto unwind double-stranded nucleic acids into two single-stranded nucleic acids. Initially, helicases were only thought to be molecular engines that unwind nucleic acids during replication, recombination, and DNA repair. Recent studies have shown that they are also involved in other biological processes, including displacement of proteins from nucleic acid, movement of Holliday junctions, chromatin remodeling, catalysis of nucleic acid conformational changes, several aspects of RNA metabolism, including transcription, mRNA splicing, mRNA export, translation, RNA stability and mitochondrial gene expression. Some human diseases, including Bloom's syndrome, Werner's syndrome, and Xeroderma Pigmentosum have been associated with defects in helicase function.,SARS-CoV 2 Helicase,SARS-CoV 2 nsp13

Molekulargewicht:

calculated_mw: 112.8 kDa

observed_mw: 120 kDa