Coronavirus Spike Glycoprotein Protein (CoV S) (AA 14-685)

Produktnummer ABIN7668597

Recombinant Coronavirus Spike Glycoprotein-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für Coronavirus Spike Glycoprotein Protein (CoV S) (AA 14-685) (ABIN7668597)

Target: Coronavirus Spike Glycoprotein (CoV S)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Applikation: ELISA

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 14-685

Verwendungszweck: Recombinant SARS-CoV-2 S1 Protein

Sequenz: Gln14-Arg685

Aufreinigung: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized Human ACE-2-Fc(Cat#PKSR030492) at 10μg/ml (100 μl/well) can bind Recombinant 2019-nCoV S1 Protein (Cat#PKSR030481). Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant 2019-nCoV S1 Protein (Cat#PKSR030481) is 1. 18 ug/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Antigendetails

Target: Coronavirus Spike Glycoprotein (CoV S)

Andere Bezeichnung: Spike

Substanzklasse: Viral Protein

Hintergrund: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.,2019-nCoV S1 protein,S1 protein,coronavirus S1 Protein,cov S1 Protein

Molekulargewicht:

calculated_mw: 78.3 kDa

observed_mw: 100-130 kDa

UniProt: P0DTC2