Adrenomedullin Protein (ADM) (His tag)

Produktnummer ABIN7668126

Recombinant Adrenomedullin-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Adrenomedullin Protein (ADM) (His tag) (ABIN7668126)

Target: Adrenomedullin (ADM)

Protein-Typ: Recombinant

Biologische Aktivität: Inactive

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 %

Produktdetails

Aufreinigungstag / Konjugat: Dieses Adrenomedullin Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Rat Adrenomedullin/ADM protein (His tag)

Sequenz: Thr 21-Leu 185

Aufreinigung: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 10 EU/mg of the protein as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: Adrenomedullin (ADM)

Andere Bezeichnung: Adrenomedullin,ADM

Substanzklasse: Hormone

Hintergrund: Adrenomedullin (ADM, also AM) is a secreted, monomeric, 6 kDa member of the Adrenomedullin family of molecules. It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes. ADM has multiple functions, including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion. The ADM preproprecursor is 185 amino acids (aa) in length. It contains a 21 aa signal sequence, a processed 20 aa peptide termed PAMP (aa 22-41), an N-terminal propeptide (aa 45-91), the ADM precursor (amidation is required for maturation) (aa 94 143), and a C-terminal propeptide (aa 150-185). The ADM precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated ADM (aa 94 143).,ADM precursor,Adrenomedullin,PAMP,Preproadrenomedullin,ProAM N terminal 20 peptide,ProAM N20

Molekulargewicht:

calculated_mw: 18.0 kDa

observed_mw: 25 kDa

UniProt: P43145

Pathways: Hormone Transport, Hormone Activity, C21-Steroid Hormone Metabolic Process, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Tube Formation