Plasmolipin Protein (DYKDDDDK Tag,Strep Tag)

Produktnummer ABIN7597048

Dieses Synthetic Nanodisc Plasmolipin-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für Plasmolipin Protein (DYKDDDDK Tag,Strep Tag) (ABIN7597048)

Target: Plasmolipin

Protein-Typ: Synthetic Nanodisc

Spezies: Human

Quelle: HEK-293 Cells

Applikation: ELISA, Cryogenic electron microscopy (cryo-EM), Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Plasmolipin Protein ist gelabelt mit DYKDDDDK Tag,Strep Tag.

Verwendungszweck: Human PLLP-Strep full length protein-synthetic nanodisc

Produktmerkmale: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations:

• Intolerant to acids and high concentrations of divalent metal ions

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please see Certificate of Analysis for specific instructions of reconstitution

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: Plasmolipin

Andere Bezeichnung: PLLP

Hintergrund: PMLP, TM4SF11
Appears to be involved in myelination. Could also participate in ion transport events as addition of plasmolipin to lipid bilayers induces the formation of ion channels, which are voltage-dependent and K( )-selective (By similarity).[UniProtKB/Swiss-Prot Function]

Molekulargewicht: The human full length PLLP-Strep protein has a MW of 20 kDa

UniProt: Q9Y342