TAS2R16 Protein (DYKDDDDK Tag,Strep Tag)

Produktnummer ABIN7596947

Dieses Synthetic Nanodisc TAS2R16-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für TAS2R16 Protein (DYKDDDDK Tag,Strep Tag) (ABIN7596947)

Target: TAS2R16 (Taste Receptor, Type 2, Member 16 (TAS2R16))

Protein-Typ: Synthetic Nanodisc

Spezies: Human

Quelle: HEK-293 Cells

Applikation: ELISA, Cryogenic electron microscopy (cryo-EM), Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Produktdetails

Aufreinigungstag / Konjugat: Dieses TAS2R16 Protein ist gelabelt mit DYKDDDDK Tag,Strep Tag.

Verwendungszweck: Human T2R16-Strep full length protein-synthetic nanodisc

Produktmerkmale: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations:

• Intolerant to acids and high concentrations of divalent metal ions

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please see Certificate of Analysis for specific instructions of reconstitution

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: TAS2R16 (Taste Receptor, Type 2, Member 16 (TAS2R16))

Andere Bezeichnung: T2R16

Hintergrund: BGLPT, T2R16
This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily. These family members are specifically expressed by taste receptor cells of the tongue and palate epithelia. Each of these apparently intronless genes encodes a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered with another 3 candidate taste receptor genes in chromosome 7 and is genetically linked to loci that influence bitter perception. [provided by RefSeq, Jul 2008]

Molekulargewicht: The human full length T2R16-Strep protein has a MW of 34 kDa

UniProt: Q9NYV7