CLDN18.2 protein (DYKDDDDK Tag,Strep Tag)

Produktnummer ABIN7596492

Synthetic Nanodisc -Protein exprimiert in HEK-293 Cells.

Kurzübersicht für CLDN18.2 protein (DYKDDDDK Tag,Strep Tag) (ABIN7596492)

Target: CLDN18.2

Protein-Typ: Synthetic Nanodisc

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Cryogenic electron microscopy (cryo-EM), ELISA, Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Produktdetails

Aufreinigungstag / Konjugat: DYKDDDDK Tag,Strep Tag

Verwendungszweck: Human CLDN18.2-Strep full length protein-synthetic nanodisc

Anwendungsinformationen

Kommentare:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins
• Mammalian cell expression system ensures post- translational modifications

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: CLDN18.2

Hintergrund: SFTA5, SFTPJ
A member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molekulargewicht: The human full length CLDN18.2-Strep Protein has a MW of 27.5 kDa

UniProt: P56856