IgE Protein

Produktnummer ABIN7596472

Dieses Recombinant IgE-Protein wird in Human produziert.

Kurzübersicht für IgE Protein (ABIN7596472)

Target: IgE

Protein-Typ: Recombinant

Spezies: Human

Quelle: Human

Applikation: Western Blotting (WB), ELISA, Isotype Control (IsoC), Functional Studies (Func), Flow Cytometry (FACS)

Reinheit: >90%, estimated from preparative Affinity Chromatography

Produktdetails

Klonalität: Chimeric

Verwendungszweck: Chimeric Human IgE (JW8)

Spezifität: 4-Hydroxy-3-nitrophenylacetyl (NP)

Produktmerkmale: Recombinant chimeric IgE consisting of a human Fc heavy chain linked to the antigen binding variable region of a mouse hybridoma by in vitro DNA recombination. The antibody is specific for the hapten 4-Hydroxy-3-iodo-5-nitrophenylacetyl (NIP). IgE (JW8) recognizes the human high affinity IgE receptor (FceRIa) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with NIP-BSA conjugate as specific cross-linking agent.

Aufreinigung: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B – mouse anti-human IgE mAb (BSW17)

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise: ELISA, Flow Cytometry, functional cellular assays (mast cell / basophil activation).

Kommentare:

100% reactivity, compared to calibrator (Internal Standard IgE (JW8)), tested by SPR (Biacore™) binding to immobilized recombinant human high affinity IgE receptor

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS) pH = 7.2

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4 °C. For long term storage, aliquot and store at -20°C or -80°C. Avoid repeated freeze-thawing. Handle under sterile conditions, the mAb is formulated in sterile buffer solution and does not contain sodium azide as preservative. The product may be shipped on ice pack or dry ice.

Antigendetails

Target: IgE