alpha-Latrotoxin Protein

Produktnummer ABIN7582344

Dieses Native alpha-Latrotoxin-Protein wird in Spider (Araneae) exprimiert.

Kurzübersicht für alpha-Latrotoxin Protein (ABIN7582344)

Target: alpha-Latrotoxin

Protein-Typ: Native

Spezies: Latrodectus tredecimguttatus

Quelle: Spider (Araneae)

Applikation: Blocking Peptide (BP)

Reinheit: ≥98 % (HPLC)

Produktdetails

Verwendungszweck: An Inducer of Neurotransmitter Release

Spezifität: Toxin target: Neurexin-1, ADGRL1 (latrophilin-1/CIRL), and tyrosine-protein phosphatase S (PTPRS) receptors, Highly disulfide bridged protein Glycosylated

Produktmerkmale: An Inducer of Neurotransmitter Release

Anwendungsinformationen

Applikationshinweise: Antigen Preadsorption Control: 150 pM - 1 nM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. Soluble in water. Once dissolved in water, add an equal volume of glycerol. Do not shake or vortex. Avoid multiple freeze-thaw cycles to maintain biological activity.

Buffer: Lyopholized in 20 mM KPO4 pH 6.8.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.

Antigendetails

Target: alpha-Latrotoxin

Hintergrund: α-Latrotoxin-Lt1a, α-LTX-Lt1a, α-LTX,α-Latrotoxin is a 130 kDa protein toxin from the black widow spider venom and is the only protein in the venom that affects mammals.1,2 Application of the toxin to presynaptic preparations induces, after a delay, a huge increase in spontaneous neurotransmitter release, which can be evaluated by measuring the post synaptic response in the form of miniature end plate potentials. This toxin is widely used to induce and study neurotransmitter release, but the molecular mechanism of its action is not fully determined.α-Latrotoxin is isolated according to a modified protocol as described by Frontali3 and Grasso4.

Molekulargewicht: 130 kDa

UniProt: P23631