Fasciculin-I Protein

Produktnummer ABIN7582328

Dieses Native Fasciculin-I-Protein wird in Snake (Serpentes) produziert.

Kurzübersicht für Fasciculin-I Protein (ABIN7582328)

Target: Fasciculin-I

Protein-Typ: Native

Spezies: Dendroaspis angusticeps

Quelle: Snake (Serpentes)

Applikation: Activity Assay (AcA), Blocking Peptide (BP)

Reinheit: ≥99 % (HPLC)

Produktdetails

Verwendungszweck: A Potent and Reversible Blocker of Acetylcholinesterase

Sequenz: TMCYSHTTTS RAILTNCGEN SCYRKSRRHP PKMVLGRGCG CPPGDDYLEV KCCTSPDKCN Y-OH

Spezifität: Toxin target: Acetylcholinesterase (AChE),
Disulfide bonds between: Cys3-Cys22, Cys17-Cys39, Cys41-Cys52 and Cys53-Cys59

Produktmerkmale: A Potent and Reversible Blocker of Acetylcholinesterase

Anwendungsinformationen

Applikationshinweise: Antigen Preadsorption Control: 1 - 10 nM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Antigendetails

Target: Fasciculin-I

Hintergrund: Fasciculin-1, Fas-1, Fas1, FAS-I, Toxin TA1,Fasciculin-I is a 61 amino acid peptidyl toxin with four disulfide bonds, isolated from the Eastern green mamba (Dendroaspis angusticeps) venom. Fasciculin-I belongs to the three finger toxin family, structurally similar to the short alpha-neurotoxins and cardiotoxins1.Fasciculin-I was shown to be a selective, potent, and reversible blocker of the mammalian acetylcholinesterase (AChE) at picomolar concentrations1. Injection of Fasciculin-I to mice causes severe generalized and long-lasting twitch (fasciculation) due to its ability to accumulate acetylcholine at the neuromuscular junction2.

Molekulargewicht: 6799 Da

UniProt: P0C1Y9