kappa-Bungarotoxin Protein

Produktnummer ABIN7582319

Dieses Native kappa-Bungarotoxin-Protein wird in Snake (Serpentes) produziert.

Kurzübersicht für kappa-Bungarotoxin Protein (ABIN7582319)

Target: kappa-Bungarotoxin

Protein-Typ: Native

Spezies: Bungarus multicinctus

Quelle: Snake (Serpentes)

Applikation: Blocking Peptide (BP)

Reinheit: ≥98 % (HPLC)

Produktdetails

Verwendungszweck: A Potent Antagonist of α3/β2 Neuronal nAChR

Sequenz: RTCLISPSST PQTCPNGQDI CFLKAQCDKF CSIRGPVIEQ GCVATCPQFR SNYRSLLCCT TDNCNH-OH

Spezifität: Toxin target: α3/β2 Neuronal nAChR,
Disulfide bonds between: Cys3-Cys21, Cys14-Cys42, Cys27-Cys31, Cys46-Cys58, Cys59-Cys64

Produktmerkmale: A Potent Antagonist of α3/β2 Neuronal nAChR

Anwendungsinformationen

Applikationshinweise: Antigen Preadsorption Control: 5 - 25 nM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Antigendetails

Target: kappa-Bungarotoxin

Hintergrund: Kappa-Bungarotoxin, Kappa-bgt, Kappa-1-bungarotoxin, Long neurotoxin 2,Neuronal bungarotoxin, nBgt, Toxin F,Kappa-bungarotoxin (κ-Bungarotoxin, κ-Bgt) is a 66 amino acid peptidyl toxin isolated from the venom of the banded krait snake, Bungarus multicinctus1,2. κ-Bgt is a postsynaptic neurotoxin that potently binds and inhibits the neuronal types of nicotinic acetylcholine receptors (nAChR). It is a selective antagonist of α3-containing and some α4-containing nAChRs subunits3.Under physiological solvent conditions, κ-Bungarotoxin exists as a non-covalent homodimer4. The dimeric nature of the toxin may play a crucial role in the binding properties of κ-Bgt to neuronal types of AChRs while losing its potency on muscle receptors4-6.Neuronal nicotinic acetylcholine receptors (nAChRs) are prototypical cation-selective, ligand-gated ion channels that mediate fast neurotransmission in the central and peripheral nervous systems. nAChRs are involved in a range of physiological and pathological functions and hence are important therapeutic targets7. The κ-neurotoxins have been valuable pharmacological tools in the study of neuronal AChRs3.

Molekulargewicht: 7265 Da

UniProt: P01398