alpha-Bungarotoxin (Active) protein (Atto 633)

Produktnummer ABIN7582317

Dieses Native alpha-Bungarotoxin-Protein wird in Bungarus multicinctus (Many-banded krait) exprimiert.

Kurzübersicht für alpha-Bungarotoxin (Active) protein (Atto 633) (ABIN7582317)

Target: alpha-Bungarotoxin

Protein-Typ: Native

Biologische Aktivität: Active

Spezies: Bungarus multicinctus

Quelle: Bungarus multicinctus (Many-banded krait)

Applikation: Fluorescence Microscopy (FM), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Produktdetails

Aufreinigungstag / Konjugat: Atto 633

Verwendungszweck: α-Bungarotoxin-ATTO Fluor-633

Sequenz: IVCHTTATSP ISAVTCPPGE NLCYRKMWCD AFCSSRGKVV ELGCAATCPS KKPYEEVTCC STDKCNPHPK QRPG-OH

Spezifität: Toxin target: α1/β1/γ/δ, α7 nAChR, GABA(A) receptor subtypes

Produktmerkmale: ATTO 633 (Ex/Em: 630/651 nm). The extent of labeling is 1-3 Molecules of dye per molecule of α-Bungarotoxin, linked via primary amines.

Biological Activity Comment: α-Bungarotoxin blocks postsynaptic neuromuscular transmission via competitive inhibition of nicotinic ACh receptors (nAChRs).

Anwendungsinformationen

Applikationshinweise: Antigen Preadsorption Control: 0.5 - 3 μM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handhabung: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light

Lagerung: -20 °C

Informationen zur Lagerung: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.

Antigendetails

Target: alpha-Bungarotoxin

Hintergrund: A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites

Molekulargewicht: ~9140 Da