alpha-Bungarotoxin Protein

Produktnummer ABIN7582311

Dieses Native -Protein wird in Snake (Serpentes) exprimiert.

Kurzübersicht für alpha-Bungarotoxin Protein (ABIN7582311)

Target: alpha-Bungarotoxin

Protein-Typ: Native

Spezies: Bungarus multicinctus

Quelle: Snake (Serpentes)

Applikation: Blocking Peptide (BP)

Reinheit: ≥99 % (HPLC)

Produktdetails

Verwendungszweck: A Potent Antagonist of α7 and Muscle nAChR and GABA(A) Receptor Subtypes

Sequenz: IVCHTTATSP ISAVTCPPGE NLCYRKMWCD AFCSSRGKVV ELGCAATCPS KKPYEEVTCC STDKCNPHPK QRPG-OH

Spezifität: Toxin target: α7, α1/β1/γ/δ nAChR, GABA(A) receptor subtypes,
Disulfide bonds between: Cys3-Cys23, Cys16-Cys44, Cys29-Cys33, Cys48-Cys59 and Cys60-Cys65

Produktmerkmale: A Potent Antagonist of α7 and Muscle nAChR and GABA(A) Receptor Subtypes

Anwendungsinformationen

Applikationshinweise: Antigen Preadsorption Control: 1 nM - 3 μM

Kommentare:

Electrophysiology

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Antigendetails

Target: alpha-Bungarotoxin

Hintergrund: Long neurotoxin 1, α-Bgtx, α-BuTX,α-Bungarotoxin isoform A31 is a 74 amino acid peptidyl toxin isolated from the venom of the banded krait snake, Bungarus multicinctus1.α-Bungarotoxin blocks postsynaptic neuromuscular transmission via competitive inhibition of nicotinic ACh receptors (nAChRs) with an IC50 of 3.5 x 10-10 M, thereby preventing the depolarizing action on postsynaptic membranes and blocking neuromuscular transmission2.The toxin is selective for α7 receptors (IC50 value of 1.6 nM) and α3/β4 receptors (IC50 value of >3 μM)3,4.α-Bungarotoxin also binds to and blocks a subset of GABAA receptors (GABAARs) that contain the GABAAR β3 subunit. In particular, α-Bungarotoxin blocks GABAARs that contain interfaces between adjacent β3 subunits5.

Molekulargewicht: 7984 Da

UniProt: P60615