MEFV Protein (AA 1-767) (His tag)

Produktnummer ABIN7565095

Dieses Recombinant MEFV-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für MEFV Protein (AA 1-767) (His tag) (ABIN7565095)

Target: MEFV (Mediterranean Fever (MEFV))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-767

Aufreinigungstag / Konjugat: Dieses MEFV Protein ist gelabelt mit His tag.

Verwendungszweck: Predefined custom protein recombinant Mefv Protein expressed in mammalian cells.

Sequenz: MAKTLGDHLL NTLEELLPYD FEKFKFKLQN TSLEKGHSKI PRGHMQMARP VKLASLLITY YGEEYAVRLT LQILRATNQR QLAEELRKAT GTEHLIEENR VGGSVQSSVE NKAKSVKVPD VPEGDGTQQN NDESDTLPSS QAEVGKGPQK KSLTKRKDQR GPESLDSQTK PWTRSTAPLY RRTQGTQSPG DKESTASAQL RRNVSSAGRL QGLYNNAPGR RESKKAEVYV YLPSGKKRPR SLEITTYSRE GEPPNSEVLP TQEETRNGSL IRMRTATLNG RTTGALEKGT GIPEHSMVLD EKTFRNMSSK TSLIGEERCP TSWTENGNGS PETTESSGET AGSILSDPEV PLSLCEKPAK TPEDPASLGQ AACEGRSQDK AVCPLCHTQE GDLRGDTCVQ SSCSCSIAPG DPKASGRCSI CFQCQGLLAR KSCEAQSPQS LPQCPRHMKQ VLLLFCEDHR EPICLICRLS LEHQGHRVRP IEEAALEYKE QIREQLERLR EMRGYVEEHR LQGDKKTDDF LKQTEIQKQK ISCPLEKLYQ LLEKQEQLFV TWLQELSQTI SKVRETYYTR VSLLDEMIEE LEAKQDQPEW DLMQDIGITL HRAKMMSASE LLDTPPGVKE KLHLLYQKSK SVEKNMQCFS EMLSSEMAFS ASDVAKWEGR QPSATQVQGL VPTVHLKCDG AHTQDCDVVF YPEREAGGSE PKDYLHPQPA QDTPELHEIH SRNNKRKFKS FLKWKPSFSR TDWRLRTCCY RDLDQAAAHP NLIFSMI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: MEFV (Mediterranean Fever (MEFV))

Andere Bezeichnung: Mefv

Hintergrund: Pyrin,FUNCTION: Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma (PubMed:34471287). Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy (By similarity). Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation (By similarity). However, it can also have a positive effect in the inflammatory pathway, acting as an innate immune sensor that triggers PYCARD/ASC specks formation, caspase-1 activation, and IL1B and IL18 production (By similarity). Together with AIM2, also acts as a mediator of pyroptosis, necroptosis and apoptosis (PANoptosis), an integral part of host defense against pathogens, in response to bacterial infection (PubMed:34471287). It is required for PSTPIP1-induced PYCARD/ASC oligomerization and inflammasome formation (By similarity). Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization (By similarity). {ECO:0000250|UniProtKB:O15553, ECO:0000269|PubMed:34471287}.

Molekulargewicht: 86.4 kDa

UniProt: Q9JJ26

AlphaFold: Q9JJ26

Pathways: Positive Regulation of Endopeptidase Activity