MCM8 Protein (AA 1-833) (His tag)

Produktnummer ABIN7564956

Recombinant MCM8-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für MCM8 Protein (AA 1-833) (His tag) (ABIN7564956)

Target: MCM8 (Minichromosome Maintenance Deficient 8 (MCM8))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-833

Aufreinigungstag / Konjugat: Dieses MCM8 Protein ist gelabelt mit His tag.

Verwendungszweck: Predefined custom protein recombinant Mcm8 Protein expressed in mammalian cells.

Sequenz: MSGAYRGRGF GRGRFQSWKR GRGGGNFSGR WRERENRVDL NEASGKHASA QASQPLLQQS TLDQFIPYKG WKLYFSEVYS NNSPFIEKIQ AFEKFFTRHI DLYDKDEIER KGSILVDFKE LTKADEITNL IPDIENALRD APEKTLACMG LAIHQVLTKD LERHAAELQA QEGLSNGGET MVNVPHIYAR VYNYEPLTHL KNIRATCYGK YISIRGTVVR VSNIKPLCTN MAFQCAACGE IQSFPLPDGK YTLPTKCPVP ACRGRSFAPL RSSPLTVTLD WQLIKIQELM SDAQREAGRI PRTIECELVH DLVDSCVPGD TVTVTGIVKV SNSEEGSRNK NDKCMFLLYI EANSVSNSKG PKAQTAEDGC KHGTLMEFSL KDLYAIREIQ AEENLLKLVV NSLCPVIFGH ELVKAGLTLA LFGGSQKYAD DKNRIPIRGD PHVLIVGDPG LGKSQMLQAA CNVAPRGVYV CGNTTTSSGL TVTLSKDSSS GDFALEAGAL VLGDQGICGI DEFDKMGNQH QALLEAMEQQ SISLAKAGVV CSLPARTSII AAANPVGGHY NKARTVSENL KMGSALLSRF DLVFILLDTP NEQHDHLLSE HVIAIRAGKQ KAVSSATVTR VLSQDSNTSV LEVVSEKPLS ERLKVAPGEQ TDPIPHQLLR KYIGYARQYV HPRLSTDAAQ ALQDFYLELR KQSQRVGSSP ITTRQLESLI RLTEARARLE LREEATREDA EDIIEIMKHS MLGTYSDEFG NLDFERSQHG SGMSNRSTAK RFISALNSIA ERTYNNIFQY HQLRQIAKEL NIQVADFENF IGSLNDQGYL LKKGPKIYQL QTM Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: MCM8 (Minichromosome Maintenance Deficient 8 (MCM8))

Andere Bezeichnung: Mcm8

Hintergrund: DNA helicase MCM8 (EC 3.6.4.12) (Minichromosome maintenance 8),FUNCTION: Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC) (By similarity). Probably by regulating HR, plays a key role during gametogenesis (PubMed:22771120). Stabilizes MCM9 protein (By similarity). {ECO:0000250|UniProtKB:Q9UJA3, ECO:0000269|PubMed:22771120}.

Molekulargewicht: 92.4 kDa

UniProt: Q9CWV1

AlphaFold: Q9CWV1

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA