EIF3C Protein (AA 1-911) (His tag)

Produktnummer ABIN7564689

Recombinant EIF3C-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für EIF3C Protein (AA 1-911) (His tag) (ABIN7564689)

Target: EIF3C (Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-911

Aufreinigungstag / Konjugat: Dieses EIF3C Protein ist gelabelt mit His tag.

Verwendungszweck: Predefined custom protein recombinant Eif3c Protein expressed in mammalian cells.

Sequenz: MSRFFTTGSD SESESSLSGE ELVTKPVSGN YGKQPLLLSE DEEDTKRVVR SAKDKRFEEL TNLIRTIRNA MKIRDVTKCL EEFELLGKAY GKAKSIVDKE GVPRFYIRIL ADLEDYLNEL WEDKEGKKKM NKNNAKALST LRQKIRKYNR DFESHITNYK QNPEQSADED AEKNEEDSEG SSDEDEDEDG VGNTTFLKKK QESSGESRKF HKKMEDDDED SEDSEDEEWD TSSTSSDSDS EEEEGKQTVL ASKFLKKAPT TEEDKKAAEK KREDKAKKKH DRKSKRLDEE EEDNEGGEWE RVRGGVPLVK EKPKMFAKGT EITHAVVIKK LNEILQVRGK KGTDRATQIE LLQLLVQIAA ENNLGVGVIV KIKFNIIASL YDYNPNLATY MKPEMWQMCL DCINELMDTL VAHSNIFVGE NILEESENLH NFDQPLRVRG CILTLVERMD EEFTKIMQNT DPHSQEYVEH LKDEAQVCAI IERVQRYLEE KGTTEEICQI YLRRILHTYY KFDYKAHQRQ LTPPEGSSKS EQDQAENEGE DSAVLMERLC KYIYAKDRTD RIRTCAILCH IYHHALHSRW YQARDLMLMS HLQDNIQHAD PPVQILYNRT MVQLGICAFR QGLTKDAHNA LLDIQSSGRA KELLGQGLLL RSLQERNQEQ EKVERRRQVP FHLHINLELL ECVYLVSAML LEIPYMAAHE SDARRRMISK QFHHQLRVGE RQPLLGPPES MREHVVAASK AMKMGDWKTC HSFIINEKMN GKVWDLFPEA DKVRTMLVRK IQEESLRTYL FTYSSVYDSI SMETLSDMFE LDLPTVHSII SKMIINEELM ASLDQPTQTV VMHRTEPTAQ QNLALQLAEK LGSLVENNER VFDHKQGTYG GYFRDQKDGY RKNEGYMRRG GYRQQQSQTA Y Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: EIF3C (Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C))

Andere Bezeichnung: Eif3c

Hintergrund: Eukaryotic translation initiation factor 3 subunit C (eIF3c) (Eukaryotic translation initiation factor 3 subunit 8) (eIF3 p110),FUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. {ECO:0000255|HAMAP-Rule:MF_03002, ECO:0000269|PubMed:17581632}.

Molekulargewicht: 105.5 kDa

UniProt: Q8R1B4

AlphaFold: Q8R1B4

Pathways: Ribonucleoprotein Complex Subunit Organization