PASK Protein (AA 1-1383) (His tag)

Produktnummer ABIN7564552

Produktdetails

Target: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1383

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PASK Protein ist gelabelt mit His tag.

Verwendungszweck: Custom-made recombinant Pask Protein expressed in mammalian cells.

Sequenz: MEDRGPPVFA EDWKCLSESP PVQEGPAAQA TFEPSKPLSI AHKHLSRKNG LSRLCQSRMA LSEDRWSSYC LSSLAAQNIC TSKLHCAAAP EYADPTAGPL GSTSCCSLLR GLASGCSGSL LSTPVCNPNK AVFTVDAKTT EILVANDKAC SLLGYSSHDL IGQKLAQFFL KSDSEVVEAL SEEHVEADGH AAVVFGTVVD IVSRIGEKIP VSVWIKRLQQ DRGLCCVVVL EPVERVSAWV AFQSDGTITS CDSLFAHLHG FTSPKDVVGQ CVIDLIPSMQ LPPPGQHIPK SLKIQRSVGR ARDGTTFPLS LKLKSKPSGR AVADSEAASE PGYQASVWVF CTISGLITLL PDGTIYGVNH SFALMLFGYG KTELLGKNIT FLIPGFYHYM DLTYDSSVQL PDLVNCLDIG RKSGPGEMNS DAQHNWELAS GAQGPRIDVV LARDHMPSQD ETLKLVGGQV SSRTQTRLET GYKILPSSAC QPSLGVDSNP EDGEQSLLTD QQSIPKRNLP AHGGQNQLDT SEISLPVLKE HLLSEIQKNI SEESPLTHRK WLSKVQQNPT KGSLPIHEEQ LLFAGQHIHV LGKEDPSAAE SYRESLLEES KSKPVDAKLF ASCEDSEPLV SVKDRGSSVD TCNLHQEAQL ELMGVSSPNP WADATMPEPH TTGQIAGGSL TYCPQYRSEW ASQQRGQDSA PSPSGMACVL LGTPTLDEPW PGVRNDREEL QTCLIKEQLS KSSCEGNLGI SRVELVPEEH PPFTAPVSFC DLGGRDLHAS RSGSSSACYA LATDLPGVLE AVEAQEADVN SYSWNLKELF LKDQTDRTPS HCSCTTSELS EAPSLSVVGS DLDVGILHRQ TSDILVDREM LLLTGTYFDL SEGQRFQEMG AGHDRAELSN ISLVSSEHYE TSDIESPGCD PPLPDPGPND MCLSAEKPRP SAQITSTPVA RGATSLQQEI QEGIYSGSCY HRDGLQLSIQ FEVKRVELQG SATLFCCWLV KDLFHSHRDS ATRTRLFLAS LPSSTHSMPE LSGSSFGEVL RAKPWFEESP TPAELEGLAA CEGEYDYKYN TISPLGSGAF GFVWTAVEKE CNKEVVVKFI KKEKVLEDCW IEDPKLGRVT LEIAILSKVD HANIIKVLDI FENQEFFQLV MEKHGSGMDL FAFIDHHPCL DEPLASFIFR QLVSAVGYLH SQGIIHRDIK DENIVIAEDF TIKLIDFGSA AYLERGKLFY TFCGTIEYCA PEVLIGNPYR GPELEMWSLG VTLYTLIFEE NPFCEVEETM EAVIHPPFLV SQELMSLLSG LLQPCPEQRT TLEKLIRDPW VTQPVNLASY TWEEVCRTNQ PESGLLSAAS LEIGSRSPSE MAQREGLCGP PAPRETRGDQ HCLHLKDPSL PVS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Andere Bezeichnung: Pask (PASK Produkte)

Hintergrund: PAS domain-containing serine/threonine-protein kinase (PAS-kinase) (PASKIN) (EC 2.7.11.1),FUNCTION: Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells, however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation. {ECO:0000269|PubMed:15148392, ECO:0000269|PubMed:17878307, ECO:0000269|PubMed:18509100, ECO:0000269|PubMed:21181396}.

Molekulargewicht: 151.3 kDa

UniProt: Q8CEE6

AlphaFold: Q8CEE6

Pathways: Regulation of Carbohydrate Metabolic Process