PPP4R2 Protein (AA 1-417) (His tag)

Produktnummer ABIN7563625

Dieses Recombinant PPP4R2-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für PPP4R2 Protein (AA 1-417) (His tag) (ABIN7563625)

Target: PPP4R2 (Protein Phosphatase 4, Regulatory Subunit 2 (PPP4R2))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-417

Aufreinigungstag / Konjugat: Dieses PPP4R2 Protein ist gelabelt mit His tag.

Verwendungszweck: Predefined custom protein recombinant Ppp4r2 Protein expressed in mammalian cells.

Sequenz: MDVERLQEAL KDFEKRGKKE VCPVLDQFLC HVAKTGETMI QWSQFKGYFI FKLEKVMDDF RTSAPEPRGP PNPNVEYIPF DEMKERILKI VTGFNGIPFT IQRLCELLTD PRRNYTGTDK FLRGVEKNVM VVSCVCPSSE KNNSNSLNRM NGVMFPGNSP NYTDRSNING PGTPRPLNRP KLSLSAPLTT NGLPESTDSK DSELQLSEEK GHSDSSASES EVSLLSPVKN KHPDEDAVES EEHEVKRLKF DKEGDVRETA SQTVSGEVSS VRAEETETAA PPPDKDRESR TRQHCTEEEE EEEEEEEEEE EESFMTPREM VPERKNQEKE SDDALTVNEE TSEESHQMEG SGVSPAQTDS TSERSDSAGA SRSGSDCLET QESGGPPSSK TGESVSVPSS MESEEATEVT DDPMEQD Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: PPP4R2 (Protein Phosphatase 4, Regulatory Subunit 2 (PPP4R2))

Andere Bezeichnung: Ppp4r2

Hintergrund: Serine/threonine-protein phosphatase 4 regulatory subunit 2,FUNCTION: Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair (By similarity). Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair (By similarity). {ECO:0000250}.

Molekulargewicht: 46.5 kDa

UniProt: Q0VGB7

AlphaFold: Q0VGB7