MPRIP Protein (AA 1-1024) (His tag)

Produktnummer ABIN7563549

Recombinant MPRIP-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für MPRIP Protein (AA 1-1024) (His tag) (ABIN7563549)

Target: MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-1024

Aufreinigungstag / Konjugat: Dieses MPRIP Protein ist gelabelt mit His tag.

Verwendungszweck: Predefined custom protein recombinant Mprip Protein expressed in mammalian cells.

Sequenz: MSAAKENPCR KFQANIFNKS KCQNCFKPRE SHLLNDEDLT QAKPIYGGWL LLAPDGTDFD NPVHRSRKWQ RRFFILYEHG LLRYALDEMP TTLPQGTINM NQCTDVVDGE ARTGQKFSLC ILTPDKEHFI RAETKEIISG WLEMLMVYPR TNKQNQKKKR KVEPPTPQEP GPAKMAVTSS SGGSSGSSSS IPSAEKVPTT KSTLWQEEMR AKDQPDGTSL SPAQSPSQSQ PPAACTPREP GLESKEDEST ISGDRVDGGR KVRVESGYFS LEKAKQDLRA EEQLPPLLSP PSPSTPHSRR SQVIEKFEAL DIEKAEHMET NMLILTTPSS DTRQGRSERR AIPRKRDFAS EAPTAPLSDA CPLSPHRRAK SLDRRSTESS MTPDLLNFKK GWLTKQYEDG QWKKHWFVLA DQSLRYYRDS VAEEAADLDG EINLSTCYDV TEYPVQRNYG FQIHTKEGEF TLSAMTSGIR RNWIQTIMKH VLPASAPDVT SSLPEGKNKS TSFETCSRST EKQEAEPGEP DPEQKKSRAR ERRREGRSKT FDWAEFRPIQ QALAQERASA VGSSDSGDPG CLEAEPGELE RERARRREER RKRFGMLDTI DGPGMEDTAL RMDIDRSPGL LGTPDLKTQN VHVEIEQRWH QVETTPLREE KQVPIAPLHL SLEDRSERLS THELTSLLEK ELEQSQKEAS DLLEQNRLLQ DQLRVALGRE QSAREGYVLQ ATCERGFAAM EETHQKKIED LQRQHQRELE KLREEKDRLL AEETAATISA IEAMKNAHRE EMERELEKSQ RSQISSINSD IEALRRQYLE ELQSVQRELE VLSEQYSQKC LENAHLAQAL EAERQALRQC QRENQELNAH NQELNNRLAA EITRLRTLLT GDGGGESTGL PLTQGKDAYE LEVLLRVKES EIQYLKQEIS SLKDELQTAL RDKKYASDKY KDIYTELSIA KAKADCDISR LKEQLKAATE ALGEKSPEGT TVSGYDIMKS KSNPDFLKKD RSCVTRQLRN IRSKSVIEQV SWDN Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))

Andere Bezeichnung: Mprip

Hintergrund: Myosin phosphatase Rho-interacting protein (Rho-interacting protein 3) (RIP3) (p116Rip),FUNCTION: Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. {ECO:0000269|PubMed:12732640, ECO:0000269|PubMed:15469989}.

Molekulargewicht: 116.4 kDa

UniProt: P97434

AlphaFold: P97434