Ube2fb (AA 1-185) protein (His tag)

Produktnummer ABIN7557299

Produktdetails

Target: Ube2fb

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-185

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: Custom-made recombinant Ube2fb Protein expressed in mammalian cells.

Sequenz: MLTLASKLKW DEDLKGSQTS VSTSDSTRRF SMRDKLLVTE VAELEANLPW TCKVHFPDPN KLHCFQLTVS PDEGYYQGGN FQFEIEVPDA YNMVPPKVKC LTKIWHPNIT ETGEICLSLL REHSADGTGW APTRTLKDVV WGLNSLFTDL LNFYDPLNIE AAEHHLQDKE DFRDKVDEYI RRYAR Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: Ube2fb

Hintergrund: NEDD8-conjugating enzyme UBE2F-B (EC 2.3.2.34) (NEDD8 carrier protein UBE2F-B) (NEDD8 protein ligase UBE2F-B) (RING-type E3 NEDD8 transferase UBE2F-B) (Ubiquitin-conjugating enzyme E2 F-B),FUNCTION: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. {ECO:0000250}.

Molekulargewicht: 21.4 kDa

UniProt: Q3UWQ3

AlphaFold: Q3UWQ3